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Image Search Results
Journal: Optica
Article Title: High-resolution Fourier light-field microscopy for volumetric multi-color live-cell imaging
doi: 10.1364/optica.419236
Figure Lengend Snippet: High-resolution Fourier light-field microscopy (HR-FLFM). (a) Experimental setup of HR-FLFM. The objective lens (OL) and the tube lens (TL) form wide-field images at the native image plane (NIP). The Fourier lens (FL) transforms the NIP to its back focal plane, where the microlens array (MLA) is positioned. The MLA segments the light field and forms three elemental images at its back focal plane on the sCMOS camera. DC, dichroic cube. CAM, camera. The inset diagram illustrates image formation through the customized MLA for emitters at different axial positions, capturing both the spatial and angular information in an uncompromised manner. (b) Axial stack projection (step size = 100 nm) of the experimental point-spread function (PSF) through the microlenses [(i)-(iii)] (effective pitch = 72.5 μm in the object space) within an axial range from −5–5 μm, as color-coded in the color scale bar. (c) Lateral displacement of each elemental PSF image [(i)-(iii)] at the camera plane as a function of the axial position, showing a good agreement with the theoretical prediction (dashed line). Scale bar: 10 μm.
Article Snippet: The back focal plane of the Fourier lens was partitioned by a customized microlens array (MLA, RPC Photonics, specified in Section 2.B ), forming elemental images by each individual microlens on an
Techniques: Microscopy